5x amv rt buffer (Promega)

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Promega 5x amv rt buffer
5x Amv Rt Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+amv+rt+buffer/5x+amv+rt+buffer/bio_rxiv__2023__09__16__558055-121-21-33
Average 90 stars, based on 1 article reviews

5x amv rt buffer - by Bioz Stars, 2026-09

90/100 stars

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Reverse Transcription:

Article Title: Increased ferritin and iron accumulation in tubers of thaxtomin A-habituated potato var. Yukon Gold somaclones with enhanced resistance to common scab
Article Snippet: The principal causal agent of potato common scab is Streptomyces scabiei.. This pathogen induces the formation of scabby lesions on the tuber surface, decreasing their value and marketability.. The best approach to reduce disease incidence and severity is to cultivate resistant potato varieties.

Article Title: Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells.
Article Snippet: Briefly, total RNA was isolated from the BV2 mouse microglial cells using RNAzolTMB (Tel-Test Inc., Friendswood, TX, USA). .. Subsequently, 2 μg of RNA and 2 μl of random hexamers (Promega, Madison, WI, USA) were combined, after which the mixture was heated at 65 ̊C for 15 min. A total of 1 μl of AMV reverse transcriptase (Promega), 5 μl of 2.5 mM dNTP (Promega), 0.5 μl of RNasin (Promega) and 8 μl of 5X AMV RT buffer (Promega) were then added to the mixture, which was then brought up to a final volume of 40 μl using diethylpyrocarbonate (DEPC)-treated water. .. The reaction mixture was then incubated at 42 ̊C for 2 h. PCR amplification was performed in a reaction mixture with a final volume of 40 μl that contained 1 μl of the appropriate cDNA, 0.5 μl of each set of primers at a concentration of 10 pM, 4 μl of 10X RT buffer, 1 μl of 2.5 mM dNTP and 0.2 U of Taq DNA polymerase (Takara, Shiga, Japan).

Article Title: Prevalence, distribution and control of potato virus Y (PVY) strains in Kenyan potato cultivars
Article Snippet: .. This was followed by adding 10 μl of reverse transcription mix consisting of 4 μl of 5X AMV RT buffer (Promega), 0.25 μl of 10 mM dNTP-mix, 0.5 μl of 2 mM DTT, 0.5 μl of 10u/μl AMV Reverse transcriptase (Promega) and 4.75 μl MQ water and incubating for 1 h at 42 °C to synthesize cDNA for the subsequent PCR process. .. The PCR mixture consisted of 5 μl of 5X Taq reaction buffer (Promega), 2.5 μl of 25 mM MgSO4, 0.5 μl of 10 mM dNTP-mix, 0.5 μl of 5u/μl Taq polymerase (Promega), 8.5 μl MQ water and 1 μl of each of the six forward and reverse PCR-primers (100 μM) (Table 1) and 2 μl of cDNA.

Polymerase Chain Reaction:

Incubation:

Article Title: The TcdE holin drives toxin secretion and virulence in Clostridioides difficile
Article Snippet: .. RNA (1μg) was incubated 10 min at 70°C with 1μg of hexamer oligonucleotide primers (p(DN)6, Roche) before adding 10 μl of 5X AMV RT buffer, 4 μl of dNTP (25mM), 1μl of RNasine (Promega) and 1μl of AMV RT enzyme (Promega) for a 50μl reaction volume. .. After 2h incubation at 37°C, the reaction was stopped by heat treatment (85°C) during 5 min. Real-time quantitative PCR was performed in 20μl reaction containing 20 ng of cDNAs, 10 μl of the SYBR PCR master mix (Life Technologies, Fisher) and 400 nM of tcdA , tcdB , or tcdR gene-specific primers ( ).

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